stap-seq screening vector Search Results


stap-seq screening vector  (Promega)
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Promega stap-seq screening vector
(a–c) Scatterplots showing the range of enhancer responsiveness at corrected aTSSs that contain exclusively TATA box, Inr, MTE, or DPE in a, random positions in b, and eTSSs in c, depicting replicate 1 versus 2 in a, and b, and sense versus antisense signals of replicate 1 in c. (d) Enhancer responsiveness according to <t>STAP-seq</t> versus luciferase induction by the zfh1 enhancer. Error bars, s.d.; n = 3. (e) Boxplot showing enhancer responsiveness for aTSSs of genes that are surrounded by 1 or 2 versus 5 or more enhancers (n = 1,325 and 139, respectively; Wilcoxon P value). Center line: median; limits: interquartile range; whiskers: 10th and 90th percentiles. (f) Heatmaps depicting enrichments for the most differentially enriched Gene Ontology (GO) categories and for defined sets of transcription factors among the 400 genes associated with the strongest or weakest eTSSs that contain exclusively TATA box, Inr, MTE, or DPE. (g) <t>Berkeley</t> <t>Drosophila</t> Genome Project (BDGP)39 in situ embryo images for genes representing the GO categories most strongly enriched near weak eTSSs.
Stap Seq Screening Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
stap-seq screening vector - by Bioz Stars, 2026-04
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(a–c) Scatterplots showing the range of enhancer responsiveness at corrected aTSSs that contain exclusively TATA box, Inr, MTE, or DPE in a, random positions in b, and eTSSs in c, depicting replicate 1 versus 2 in a, and b, and sense versus antisense signals of replicate 1 in c. (d) Enhancer responsiveness according to STAP-seq versus luciferase induction by the zfh1 enhancer. Error bars, s.d.; n = 3. (e) Boxplot showing enhancer responsiveness for aTSSs of genes that are surrounded by 1 or 2 versus 5 or more enhancers (n = 1,325 and 139, respectively; Wilcoxon P value). Center line: median; limits: interquartile range; whiskers: 10th and 90th percentiles. (f) Heatmaps depicting enrichments for the most differentially enriched Gene Ontology (GO) categories and for defined sets of transcription factors among the 400 genes associated with the strongest or weakest eTSSs that contain exclusively TATA box, Inr, MTE, or DPE. (g) Berkeley Drosophila Genome Project (BDGP)39 in situ embryo images for genes representing the GO categories most strongly enriched near weak eTSSs.

Journal: Nature biotechnology

Article Title: Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution

doi: 10.1038/nbt.3739

Figure Lengend Snippet: (a–c) Scatterplots showing the range of enhancer responsiveness at corrected aTSSs that contain exclusively TATA box, Inr, MTE, or DPE in a, random positions in b, and eTSSs in c, depicting replicate 1 versus 2 in a, and b, and sense versus antisense signals of replicate 1 in c. (d) Enhancer responsiveness according to STAP-seq versus luciferase induction by the zfh1 enhancer. Error bars, s.d.; n = 3. (e) Boxplot showing enhancer responsiveness for aTSSs of genes that are surrounded by 1 or 2 versus 5 or more enhancers (n = 1,325 and 139, respectively; Wilcoxon P value). Center line: median; limits: interquartile range; whiskers: 10th and 90th percentiles. (f) Heatmaps depicting enrichments for the most differentially enriched Gene Ontology (GO) categories and for defined sets of transcription factors among the 400 genes associated with the strongest or weakest eTSSs that contain exclusively TATA box, Inr, MTE, or DPE. (g) Berkeley Drosophila Genome Project (BDGP)39 in situ embryo images for genes representing the GO categories most strongly enriched near weak eTSSs.

Article Snippet: STAP-seq screening vector For STAP-seq in Drosophila cells we constructed a screening vector based on the pGL3-Promoter backbone (Promega; cat. no. E1751) by replacing the sequence between BglII and FseI with the following sequence, containing a ccdB suicide gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc), followed by the pGL3′s SV40 late polyA-signal.

Techniques: Luciferase, In Situ